Journal: iScience

Article Title: Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet

doi: 10.1016/j.isci.2022.104184

Figure Lengend Snippet:

Article Snippet: Plasmid: pCMV Flag ERR1 , Addgene , #10975.

Techniques: Marker, Extraction, Transfection, Lysis, Luciferase, Protease Inhibitor, RNA Sequencing, Recombinant, Plasmid Preparation, Software

Journal: Plants (Basel, Switzerland)

Article Title: Enhancing Maize Transformation and Targeted Mutagenesis through the Assistance of Non-Integrating Wus2 Vector.

doi: 10.3390/plants12152799

Figure Lengend Snippet: Figure 1. NIW-assisted transformation. (a) Schematic illustration of NIW-assisted maize transforma- tion. Agrobacterium cells harboring GOI and NIW constructs are mixed at a 9:1 ratio before immature embryo infection, and Wus2 protein diffuses to the neighboring cells through the plasmodesmata and stimulates cell proliferation, promoting the regeneration of cells without Wus2 integration. (b) T-DNA region of NIW construct pKL2391. (c) T-DNA region of GOI construct pKL2359. LB and RB, left and right T-DNA border sequences; P35S-RUBY, RUBY reporter consisting of betalain biosynthesis genes (CYP76AD1, DODA, and Glucosyl transferase) driven by CaMV 35S promoter and Arabidopsis heat shock protein 18.2 gene terminator; 3×ENH-PzmUbi-ZmWUS2, maize Wus2 expression cassette driven by 3× viral enhancers, maize ubiquitin promoter, and In2-1 gene terminator; P35S-mCherry, a red fluorescent protein (mCherry) expression cassette driven by CaMV 35S promoter and soybean vegetative storage protein terminator; PzmUbi-SpCas9, maize codon optimized Cas9 from Streptococ- cus pyogenes (SpCas9) with maize ubiquitin promoter and rbcS-E9 gene terminator; PosU3-gRNA, a single-guide RNA targeting maize Glossy2 gene driven by rice U3 promoter; PzmUbi-NptII, neomycin phosphotransferase II (NptII) gene driven by maize ubiquitin promoter and potato proteinase inhibitor II gene terminator.

Article Snippet: LBA4404Thystrain was used for the GOI construct pKL2359 (Figure 1c, Addgene#199721; [12]) in all experiments.

Techniques: Transformation Assay, Construct, Infection, Expressing, Ubiquitin Proteomics

Journal: iScience

Article Title: YAP regulates alveolar epithelial cell differentiation and AGER via NFIB/KLF5/NKX2-1

doi: 10.1016/j.isci.2021.102967

Figure Lengend Snippet:

Article Snippet: Plasmids used were acquired as follows: pCAGG:NFIB2:HA (112700 Addgene), pCDNA:KLF5 , pCMV-flag (S127A)YAP (27370 Addgene) , pCDNA:NKX2-1 , pGL3:KLF5:Luciferase, pLenti:AGER:Luciferase (ABM).

Techniques: Generated, Recombinant